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Cowgirl Uniqua is a horse rider and clarinetist. In "Riding the Range", she rides a horse named Pal and searches for a mysterious bandit. In "Polka Palace Party", she helps Cowboy Tyrone form a new polka band called the Ramblin' Polka Cowboys.
Cowgirl Uniqua is mostly pink. She wears a pair of pink overalls, a lilac-colored bandana, and a tan hat.